Review



pbluescript sk1 (pbssk  (Agilent technologies)


Bioz Verified Symbol Agilent technologies is a verified supplier
Bioz Manufacturer Symbol Agilent technologies manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Agilent technologies pbluescript sk1 (pbssk
    Pbluescript Sk1 (Pbssk, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+sk1+(pbssk/pm25395220-50-15-19
    Average 90 stars, based on 1 article reviews
    pbluescript sk1 (pbssk - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Uncovering methods for the prevention of protein aggregation and improvement of product quality in a transient expression system.
    Article Snippet: Expression vector pTT5 is licensed from the National Research Council of Canada (NRCC).4,7 pTT5 and pBluescript SK1 (pBSSK) vectors (Agilent Technologies, Santa Clara, CA) lacking a coding DNA insert, and sheared salmon sperm (ssDNA) (Thermo Scientific, Waltman, MA) were used as carrier DNA.

    Article Title: Evidence for impaired retinoic acid receptor-thyroid hormone receptor AF-2 cofactor activity in human lung cancer
    Article Snippet: RARb promoter constructs were derived from human RARb2 promoter sequences contained within plasmid pD1 (19). pD1 was digested with BglII-BamHI, and the fragment spanning2747 to1156 of the RARb2 promoter was ligated into the BamHI site of pBluescript II SK1 (pBSSK) (Stratagene) to generate pB747. pX60 was constructed by SmaI-SacI digestion of pB747 and ligation of the fragment spanning 260 to 1156 of the RARb2 promoter into the SmaI-SacI sites of the luciferase vector pXP2 (97). pB5 was generated by removal of the 903-bp insert in pB747 by EcoRI digestion and replacement with the 5-kb EcoRI promoter fragment from pD1. pX5 was made by ligating the 5-kb HindIII-SacI fragment from pB5 into the HindIII-SacI sites of pXP2. pbRAREtk was made by annealing the oligonucleotides 59-AGCTGGGTAGG GTTCACCGAAAGTTCACTCG-39 and 59-TCGACGAGTGAACTTTCGGT GAACCCTACCC-39 and ligating the resulting duplex into the HindIII-XhoI sites of pT109luc (97).

    Plasmid Preparation:

    Article Title: Uncovering methods for the prevention of protein aggregation and improvement of product quality in a transient expression system.
    Article Snippet: Expression vector pTT5 is licensed from the National Research Council of Canada (NRCC).4,7 pTT5 and pBluescript SK1 (pBSSK) vectors (Agilent Technologies, Santa Clara, CA) lacking a coding DNA insert, and sheared salmon sperm (ssDNA) (Thermo Scientific, Waltman, MA) were used as carrier DNA.

    Article Title: Evidence for impaired retinoic acid receptor-thyroid hormone receptor AF-2 cofactor activity in human lung cancer
    Article Snippet: RARb promoter constructs were derived from human RARb2 promoter sequences contained within plasmid pD1 (19). pD1 was digested with BglII-BamHI, and the fragment spanning2747 to1156 of the RARb2 promoter was ligated into the BamHI site of pBluescript II SK1 (pBSSK) (Stratagene) to generate pB747. pX60 was constructed by SmaI-SacI digestion of pB747 and ligation of the fragment spanning 260 to 1156 of the RARb2 promoter into the SmaI-SacI sites of the luciferase vector pXP2 (97). pB5 was generated by removal of the 903-bp insert in pB747 by EcoRI digestion and replacement with the 5-kb EcoRI promoter fragment from pD1. pX5 was made by ligating the 5-kb HindIII-SacI fragment from pB5 into the HindIII-SacI sites of pXP2. pbRAREtk was made by annealing the oligonucleotides 59-AGCTGGGTAGG GTTCACCGAAAGTTCACTCG-39 and 59-TCGACGAGTGAACTTTCGGT GAACCCTACCC-39 and ligating the resulting duplex into the HindIII-XhoI sites of pT109luc (97).

    Construct:

    Article Title: Uncovering methods for the prevention of protein aggregation and improvement of product quality in a transient expression system.
    Article Snippet: Expression vector pTT5 is licensed from the National Research Council of Canada (NRCC).4,7 pTT5 and pBluescript SK1 (pBSSK) vectors (Agilent Technologies, Santa Clara, CA) lacking a coding DNA insert, and sheared salmon sperm (ssDNA) (Thermo Scientific, Waltman, MA) were used as carrier DNA.

    Article Title: Evidence for impaired retinoic acid receptor-thyroid hormone receptor AF-2 cofactor activity in human lung cancer
    Article Snippet: RARb promoter constructs were derived from human RARb2 promoter sequences contained within plasmid pD1 (19). pD1 was digested with BglII-BamHI, and the fragment spanning2747 to1156 of the RARb2 promoter was ligated into the BamHI site of pBluescript II SK1 (pBSSK) (Stratagene) to generate pB747. pX60 was constructed by SmaI-SacI digestion of pB747 and ligation of the fragment spanning 260 to 1156 of the RARb2 promoter into the SmaI-SacI sites of the luciferase vector pXP2 (97). pB5 was generated by removal of the 903-bp insert in pB747 by EcoRI digestion and replacement with the 5-kb EcoRI promoter fragment from pD1. pX5 was made by ligating the 5-kb HindIII-SacI fragment from pB5 into the HindIII-SacI sites of pXP2. pbRAREtk was made by annealing the oligonucleotides 59-AGCTGGGTAGG GTTCACCGAAAGTTCACTCG-39 and 59-TCGACGAGTGAACTTTCGGT GAACCCTACCC-39 and ligating the resulting duplex into the HindIII-XhoI sites of pT109luc (97).

    Derivative Assay:

    Article Title: Uncovering methods for the prevention of protein aggregation and improvement of product quality in a transient expression system.
    Article Snippet: Expression vector pTT5 is licensed from the National Research Council of Canada (NRCC).4,7 pTT5 and pBluescript SK1 (pBSSK) vectors (Agilent Technologies, Santa Clara, CA) lacking a coding DNA insert, and sheared salmon sperm (ssDNA) (Thermo Scientific, Waltman, MA) were used as carrier DNA.

    Article Title: Evidence for impaired retinoic acid receptor-thyroid hormone receptor AF-2 cofactor activity in human lung cancer
    Article Snippet: RARb promoter constructs were derived from human RARb2 promoter sequences contained within plasmid pD1 (19). pD1 was digested with BglII-BamHI, and the fragment spanning2747 to1156 of the RARb2 promoter was ligated into the BamHI site of pBluescript II SK1 (pBSSK) (Stratagene) to generate pB747. pX60 was constructed by SmaI-SacI digestion of pB747 and ligation of the fragment spanning 260 to 1156 of the RARb2 promoter into the SmaI-SacI sites of the luciferase vector pXP2 (97). pB5 was generated by removal of the 903-bp insert in pB747 by EcoRI digestion and replacement with the 5-kb EcoRI promoter fragment from pD1. pX5 was made by ligating the 5-kb HindIII-SacI fragment from pB5 into the HindIII-SacI sites of pXP2. pbRAREtk was made by annealing the oligonucleotides 59-AGCTGGGTAGG GTTCACCGAAAGTTCACTCG-39 and 59-TCGACGAGTGAACTTTCGGT GAACCCTACCC-39 and ligating the resulting duplex into the HindIII-XhoI sites of pT109luc (97).

    Ligation:

    Article Title: Uncovering methods for the prevention of protein aggregation and improvement of product quality in a transient expression system.
    Article Snippet: Expression vector pTT5 is licensed from the National Research Council of Canada (NRCC).4,7 pTT5 and pBluescript SK1 (pBSSK) vectors (Agilent Technologies, Santa Clara, CA) lacking a coding DNA insert, and sheared salmon sperm (ssDNA) (Thermo Scientific, Waltman, MA) were used as carrier DNA.

    Article Title: Evidence for impaired retinoic acid receptor-thyroid hormone receptor AF-2 cofactor activity in human lung cancer
    Article Snippet: RARb promoter constructs were derived from human RARb2 promoter sequences contained within plasmid pD1 (19). pD1 was digested with BglII-BamHI, and the fragment spanning2747 to1156 of the RARb2 promoter was ligated into the BamHI site of pBluescript II SK1 (pBSSK) (Stratagene) to generate pB747. pX60 was constructed by SmaI-SacI digestion of pB747 and ligation of the fragment spanning 260 to 1156 of the RARb2 promoter into the SmaI-SacI sites of the luciferase vector pXP2 (97). pB5 was generated by removal of the 903-bp insert in pB747 by EcoRI digestion and replacement with the 5-kb EcoRI promoter fragment from pD1. pX5 was made by ligating the 5-kb HindIII-SacI fragment from pB5 into the HindIII-SacI sites of pXP2. pbRAREtk was made by annealing the oligonucleotides 59-AGCTGGGTAGG GTTCACCGAAAGTTCACTCG-39 and 59-TCGACGAGTGAACTTTCGGT GAACCCTACCC-39 and ligating the resulting duplex into the HindIII-XhoI sites of pT109luc (97).

    Luciferase:

    Article Title: Uncovering methods for the prevention of protein aggregation and improvement of product quality in a transient expression system.
    Article Snippet: Expression vector pTT5 is licensed from the National Research Council of Canada (NRCC).4,7 pTT5 and pBluescript SK1 (pBSSK) vectors (Agilent Technologies, Santa Clara, CA) lacking a coding DNA insert, and sheared salmon sperm (ssDNA) (Thermo Scientific, Waltman, MA) were used as carrier DNA.

    Article Title: Evidence for impaired retinoic acid receptor-thyroid hormone receptor AF-2 cofactor activity in human lung cancer
    Article Snippet: RARb promoter constructs were derived from human RARb2 promoter sequences contained within plasmid pD1 (19). pD1 was digested with BglII-BamHI, and the fragment spanning2747 to1156 of the RARb2 promoter was ligated into the BamHI site of pBluescript II SK1 (pBSSK) (Stratagene) to generate pB747. pX60 was constructed by SmaI-SacI digestion of pB747 and ligation of the fragment spanning 260 to 1156 of the RARb2 promoter into the SmaI-SacI sites of the luciferase vector pXP2 (97). pB5 was generated by removal of the 903-bp insert in pB747 by EcoRI digestion and replacement with the 5-kb EcoRI promoter fragment from pD1. pX5 was made by ligating the 5-kb HindIII-SacI fragment from pB5 into the HindIII-SacI sites of pXP2. pbRAREtk was made by annealing the oligonucleotides 59-AGCTGGGTAGG GTTCACCGAAAGTTCACTCG-39 and 59-TCGACGAGTGAACTTTCGGT GAACCCTACCC-39 and ligating the resulting duplex into the HindIII-XhoI sites of pT109luc (97).

    Generated:

    Article Title: Uncovering methods for the prevention of protein aggregation and improvement of product quality in a transient expression system.
    Article Snippet: Expression vector pTT5 is licensed from the National Research Council of Canada (NRCC).4,7 pTT5 and pBluescript SK1 (pBSSK) vectors (Agilent Technologies, Santa Clara, CA) lacking a coding DNA insert, and sheared salmon sperm (ssDNA) (Thermo Scientific, Waltman, MA) were used as carrier DNA.

    Article Title: Evidence for impaired retinoic acid receptor-thyroid hormone receptor AF-2 cofactor activity in human lung cancer
    Article Snippet: RARb promoter constructs were derived from human RARb2 promoter sequences contained within plasmid pD1 (19). pD1 was digested with BglII-BamHI, and the fragment spanning2747 to1156 of the RARb2 promoter was ligated into the BamHI site of pBluescript II SK1 (pBSSK) (Stratagene) to generate pB747. pX60 was constructed by SmaI-SacI digestion of pB747 and ligation of the fragment spanning 260 to 1156 of the RARb2 promoter into the SmaI-SacI sites of the luciferase vector pXP2 (97). pB5 was generated by removal of the 903-bp insert in pB747 by EcoRI digestion and replacement with the 5-kb EcoRI promoter fragment from pD1. pX5 was made by ligating the 5-kb HindIII-SacI fragment from pB5 into the HindIII-SacI sites of pXP2. pbRAREtk was made by annealing the oligonucleotides 59-AGCTGGGTAGG GTTCACCGAAAGTTCACTCG-39 and 59-TCGACGAGTGAACTTTCGGT GAACCCTACCC-39 and ligating the resulting duplex into the HindIII-XhoI sites of pT109luc (97).



    Similar Products

    90
    Agilent technologies pbluescript sk1 (pbssk
    Pbluescript Sk1 (Pbssk, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+sk1+(pbssk/pm25395220-50-15-19
    Average 90 stars, based on 1 article reviews
    pbluescript sk1 (pbssk - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies pbluescript ii sk1 (pbssk)
    Pbluescript Ii Sk1 (Pbssk), supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+sk1+(pbssk/10__1128_slash_mcb__15__7__3945-78-36-40
    Average 90 stars, based on 1 article reviews
    pbluescript ii sk1 (pbssk) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results